Serial Dilution Calculator

Serial Dilution Calculator

What is this calculator used for?

This Serial Dilution Calculator helps you compute the final concentration of a solution after a series of stepwise dilutions. It's widely used in biology and chemistry, particularly in microbiology experiments, assays, and titrations.

How to use this calculator

  1. Enter the initial concentration of your stock solution (C₀).
  2. Provide the dilution factor (D), such as 10 for a tenfold dilution.
  3. Enter the number of dilution steps (n) to be performed.
  4. The final concentration after n dilutions will be calculated automatically.

Formula

Final Concentration (Cₙ) = C₀ / Dⁿ

Example

If you start with a solution of 100 mg/mL, perform 4 tenfold (D = 10) serial dilutions, the final concentration will be:

Cₙ = 100 / 10⁴ = 100 / 10,000 = 0.01 mg/mL